Hoefer SE215 Uživatelský manuál stáhnout pdf (Strana 8)

Prepare the monomer solution

Prepare enough monomer solution to ﬁll all sand-

wiches to the level of the notched plate (or lower

if a stacking gel or ﬁrst dimension IPG strip will be

added — see step 2). The formula below allows extra

solution to ﬁll the space between the sandwich and

the chamber.

•

Table 2: Variables for calculating monomer volume

gel caster area X cm

extra vol. required (Y ml)

SE215 60 11

SE235 80 8

SE275 60 5

Monomer = area X × spacer × total gel + extra chamber

vol (ml) (cm

) thickness (cm) sandwiches vol (Y ml)

An example volume calculation for the SE215 for 10 sandwiches, each with a thickness of

1.5 mm follows: From Table 2, the area variable for the SE215 is 60 and the extra volume

variable is 11 ml.

(60 cm

) (0.15 cm) (10 sandwiches) + 11 ml extra volume required = 101 ml

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Hoefer SE215 Uživatelský manuál Strana 8