Hoefer SE215 Uživatelský manuál stáhnout pdf (Strana 11)

Linear gradient gels

Gradient gels are prepared by ﬁrst pumping the

“light” (lower % acrylamide) solutions and then

gradually adding a “heavy” (higher % acryl-

amide) into the caster by way of the inlet port

on the faceplate.

Follow instructions for preparing the gel caster on

page 2. Remove only the triangular rubber plug from

the bottom of the casting chamber, leaving the two

smaller plugs in place.

Follow the instructions for constructing the gel sand-

wich stack and loading it into the caster on page 3.

Connect the gradient maker to the gel caster. Run

a length of clear vinyl tubing through a peristaltic

pump and attach one end of the tubing to the gradi-

ent maker outlet port and the other end to the casting

chamber inlet port on the faceplate. Adjust the pump

to run a rate of about 5 ml/min for 4-gel casters and

about 10 ml/min for 10-gel casters.

•

Fig 5. Gradient maker set-up.

Attach tubing to inlet port of the

multiple gel caster, run the tubing

through a peristaltic pump, and

attach to gradient maker.

Multiple gel caster

Peristaltic pump Magnetic stirrer

Mixing chamber

Gradient maker

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Hoefer SE215 Uživatelský manuál Strana 11